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Magnetic-activated cell sorting
Magnetic-activated cell sorting (MACS) is a method for separation of various cell populations depending on their surface antigens (CD molecules) invented by Miltenyi Biotec. The name MACS is a registered trademark of the company.
The method is performed using Miltenyi Biotec's MACS Technology, which uses superparamagnetic nanoparticles and columns. Magnetic-activated cell sorting is a commonly used method in areas like immunology, cancer research, neuroscience, and stem cell research.
==Procedure==
The MACS method allows cells to be separated by incubating with magnetic nanoparticles coated with antibodies against a particular surface antigen. This causes the cells expressing this antigen to attach to the magnetic nanoparticles. Afterwards the cell solution is transferred on a column placed in a strong magnetic field. In this step, the cells attached to the nanoparticles (expressing the antigen) stay on the column, while other cells (not expressing the antigen) flow through. With this method, the cells can be separated positively or negatively with respect to the particular antigen(s).
抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』
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